What Are the Challenges in Studying CYP3A4 Catalysis?
One of the primary challenges in studying CYP3A4 catalysis is its broad substrate specificity and complex regulatory mechanisms. This makes it difficult to predict the enzyme’s behavior with new substrates. Additionally, the enzyme is membrane-bound, which complicates its isolation and characterization. Researchers often use recombinant systems and advanced analytical techniques to study CYP3A4’s catalytic properties in vitro.
