Despite its importance, the catalytic conversion of D-glucose to L-sorbose is not without challenges. One major issue is the need for precise control over the fermentation conditions to ensure high yield and purity of L-sorbose. Variability in microbial activity can lead to inconsistent results, necessitating strict monitoring and optimization. Furthermore, the downstream chemical steps required to convert L-sorbose to Vitamin C often involve harsh conditions and hazardous reagents, prompting the need for safer alternatives.
