What Are Some Experimental Techniques Used to Study Sodium-Potassium ATPase?
1. Crystallography: X-ray crystallography has been instrumental in revealing the pump's structure. 2. Electrophysiology: Techniques like patch-clamp can measure ionic currents and help in understanding the pump's activity. 3. Molecular Dynamics Simulations: These simulations provide insights into the dynamic aspects of the enzyme's function. 4. Mutagenesis Studies: These studies involve altering specific amino acids to understand their role in the enzyme's mechanism.