Performing an assay typically involves the following steps:
1. Preparation: Select the appropriate reactants, solvents, and conditions for the reaction. 2. Execution: Carry out the reaction under controlled conditions while maintaining precise measurements of time, temperature, and other variables. 3. Analysis: Use analytical techniques such as gas chromatography (GC), high-performance liquid chromatography (HPLC), or spectroscopy to quantify the products and reactants. 4. Interpretation: Analyze the data to calculate the activity, selectivity, and stability of the catalyst.