Researchers study TPP in catalysis using various biochemical and structural biology techniques. X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy are commonly used to elucidate the structure of TPP-bound enzymes. Kinetic studies help understand the reaction mechanisms and the role of TPP in stabilizing reaction intermediates. Computational methods, such as molecular dynamics simulations, are also employed to model the interactions between TPP and enzymes at the atomic level.
