Producing recombinant glucocerebrosidase involves several steps:
Gene Cloning: The gene encoding glucocerebrosidase is isolated and inserted into a suitable expression vector. Transformation: The vector is introduced into a host organism, often Chinese Hamster Ovary (CHO) cells or yeast, which will express the enzyme. Fermentation: The host cells are cultured in bioreactors to produce large quantities of the enzyme. Purification: The enzyme is extracted and purified to obtain the recombinant glucocerebrosidase.
