The activity of NOS is tightly regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational modifications. For example, eNOS activity is modulated by phosphorylation and interactions with proteins like caveolin-1 and calmodulin. The availability of cofactors and substrates, as well as the presence of inhibitors such as asymmetric dimethylarginine (ADMA), also influence NOS activity.
