Anabolic pathways are tightly regulated to maintain cellular homeostasis. Regulation can occur at multiple levels: - Gene Expression: The synthesis of enzymes involved in anabolic pathways can be regulated at the transcriptional and translational levels. - Allosteric Regulation: Enzymes can be regulated by molecules that bind to sites other than the active site, causing conformational changes that affect activity. - Post-Translational Modifications: Enzymes may be activated or deactivated through modifications such as phosphorylation or ubiquitination.