The basic principle of HPLC involves injecting a liquid sample into a stream of solvent (mobile phase) that passes through a column packed with a solid adsorbent (stationary phase). The interactions between the sample components and the stationary phase cause them to elute at different times, allowing for their separation. Detection methods such as UV/Vis spectroscopy, fluorescence, or mass spectrometry are used to identify and quantify the separated components.
