In enzyme catalysis, the folding and structural dynamics of the enzyme are critical to its function. CD spectroscopy is widely used to study these aspects by analyzing the secondary structure of enzymes. The technique can monitor changes in the enzyme's conformation upon substrate binding, during the catalytic process, and in response to environmental factors such as pH and temperature. Furthermore, CD spectroscopy can be used to assess the stability of enzymes and to screen for potential inhibitors by observing how these molecules affect the enzyme's structure.
