The RI detector operates based on the principle that light changes direction when it passes from one medium to another with different refractive indices. The detector measures the angle of this change to determine the refractive index of the sample. When a sample passes through the detector's flow cell, it alters the refractive index of the medium, causing a change in the detector's output signal. This signal can then be correlated with the concentration of the analyte in the sample.
