The active site of hydrogenases typically contains metal clusters that facilitate the binding and activation of H2. In the case of [NiFe]-hydrogenases, the active site comprises a nickel ion and an iron ion coordinated by various ligands. For [FeFe]-hydrogenases, the active site contains a diiron cluster. The enzyme undergoes conformational changes that enable the efficient transfer of electrons, facilitating the reversible conversion of H2 to protons and electrons.
