transition state

What Techniques are Used to Study the Transition State?

Several experimental and computational techniques are used to study the transition state:
1. X-ray Crystallography: Can sometimes capture an enzyme in a conformation close to the transition state.
2. Spectroscopy: Methods like NMR and IR spectroscopy can provide information about the structure of the transition state.
3. Computational Chemistry: Quantum mechanical calculations can predict the structure and energy of the transition state, providing valuable insights that are often difficult to obtain experimentally.

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