What Techniques Are Used to Study Proofreading in Catalysis?
Several advanced techniques are employed to study proofreading mechanisms in catalysis, including:
X-ray crystallography: Provides detailed structural information about the active sites and proofreading sites of enzymes. NMR spectroscopy: Helps in understanding the dynamics and interactions within the catalytic and proofreading sites. Kinetic assays: Used to measure the rates of correct and incorrect substrate processing, providing insights into the efficiency of proofreading mechanisms.
