Various techniques are employed in metagenomics, including:
DNA Extraction: The first step involves extracting DNA from environmental samples. Sequencing: High-throughput sequencing technologies, such as Illumina and PacBio, are used to sequence the extracted DNA. Bioinformatics: Advanced computational tools are used to assemble and analyze the sequenced data, identifying genes and predicting their functions. Functional Screening: The identified genes are cloned and expressed in suitable hosts to screen for catalytic activity.
