- Allosteric Regulation: The binding of molecules at secondary sites can induce conformational changes in the enzyme, altering its activity. This is known as allosteric regulation. - Substrate Channeling: Secondary binding sites can facilitate the transfer of intermediates between multiple catalytic sites, a process known as substrate channeling. - Enhanced Selectivity and Stability: By binding to certain molecules, the secondary site can stabilize the enzyme's conformation and enhance its selectivity for specific substrates or reactions.