Several parameters need calibration, including: 1. Temperature: Ensuring the reaction occurs at an optimal temperature. 2. Pressure: Adjusting the pressure levels to favor specific reactions. 3. Flow Rates: Controlling the flow of reactants and products. 4. Concentration: Calibrating the concentration of reactants to achieve the desired reaction pathway. 5. pH Levels: In biochemical catalysis, pH can play a critical role in enzyme activity.