The catalytic mechanism of phosphorylases involves several steps: 1. Substrate Binding: The substrate molecule binds to the active site of the enzyme. 2. Phosphate Group Transfer: The enzyme facilitates the transfer of a phosphate group from inorganic phosphate to the substrate. 3. Product Release: The resultant phosphorylated product is released, and the enzyme is free to catalyze another reaction cycle.
This entire process is highly dependent on the structural integrity of the enzyme and the precise alignment of catalytic residues.
