SpCas9 HF1 is a high-fidelity variant of the widely used CRISPR-Cas9 genome-editing tool, designed to minimize off-target effects while retaining robust on-target activity. The "HF1" suffix stands for "High Fidelity 1," indicating its enhanced precision. This variant was engineered by introducing specific mutations to the original SpCas9 enzyme, reducing the likelihood of unintended genetic modifications.
