What Challenges are Associated with Inhibition Constants?
Several challenges can complicate the determination and interpretation of inhibition constants:
- Complex Kinetics: Enzymes and catalytic reactions can exhibit complex kinetics that do not fit simple models, complicating Ki determination. - Multiple Binding Sites: Some enzymes have multiple binding sites, each with its own Ki, requiring more sophisticated analysis. - Experimental Conditions: Variations in pH, temperature, and ionic strength can affect enzyme activity and inhibitor binding, leading to variability in Ki values.
