Despite its advantages, HAADF also has some limitations:
Sample Preparation: Preparing samples for HAADF can be challenging and may introduce artifacts. Radiation Damage: High-energy electron beams can cause damage to sensitive materials, potentially altering their structure. Limited Penetration: HAADF is less effective for thick samples due to limited electron penetration. Cost and Complexity: The equipment and expertise required for HAADF are expensive and complex, limiting its accessibility.