Despite its many advantages, 13C NMR has some limitations:
Sensitivity: 13C NMR is less sensitive than 1H NMR due to the lower natural abundance of the carbon-13 isotope (approximately 1.1%). This can make it challenging to detect low concentrations of carbon-containing species. Resolution: Complex mixtures and overlapping signals can complicate the interpretation of 13C NMR spectra. Time Consumption: Acquiring high-quality 13C NMR spectra can be time-consuming, especially for samples with low concentrations of carbon-13.
