Several methods are employed in bioconjugation, each with its advantages and limitations. The most common methods include:
Covalent bonding: This involves the formation of stable bonds between the enzyme and the synthetic molecule. Non-covalent interactions: These include hydrogen bonding, van der Waals forces, and electrostatic interactions, which can be used for reversible conjugation. Click chemistry: A versatile and highly efficient method for bioconjugation, often used for attaching small molecules to proteins.
