Acid stable enzymes are specialized proteins that maintain their catalytic activity in highly acidic environments (pH Mechanism of Action The mechanism of action of acid stable enzymes involves the stabilization of their active sites and overall three-dimensional structure in acidic conditions. This is often achieved through a higher proportion of acidic amino acids, salt bridges, and hydrogen bonds that resist denaturation. Additionally, some acid stable enzymes have evolved to maintain a neutral microenvironment around their active sites, ensuring efficient catalysis.