Km is typically determined through experimental methods such as the Michaelis-Menten equation. This involves plotting the reaction rate (V) against substrate concentration ([S]) and fitting the data to the Michaelis-Menten equation: \[ V = \frac{V_{max} [S]}{K_m + [S]} \] From this plot, Km can be derived as the substrate concentration at which V is half of Vmax.
