Enzyme efficiency is often quantified using two key parameters: turnover number (kcat) and the Michaelis-Menten constant (Km). The turnover number represents the maximum number of substrate molecules converted to product per enzyme molecule per unit time. The Michaelis-Menten constant indicates the substrate concentration at which the reaction rate is half of its maximum value. The ratio of kcat/Km is a useful measure of enzyme efficiency, indicating how efficiently an enzyme converts substrate to product at low substrate concentrations.
