In catalysis research, TIRFM can be used to study various aspects, such as adsorption, desorption, and the movement of reactants and products on the catalyst surface. The process typically involves the following steps:
1. Sample Preparation: A thin film of the catalyst is prepared on a glass substrate. 2. Fluorophore Labeling: Reactants or intermediate species are labeled with fluorescent markers. 3. Total Internal Reflection: A laser beam is directed at the glass-liquid interface at an angle greater than the critical angle, resulting in total internal reflection and the generation of an evanescent wave. 4. Fluorescence Excitation: The evanescent wave excites the fluorophores within the thin region near the interface. 5. Imaging: The emitted fluorescence is captured using a highly sensitive camera, allowing real-time observation of molecular events.
