In RP-HPLC, the stationary phase is typically composed of hydrophobic materials such as C18 or C8 chains bonded to silica particles. The mobile phase consists of a mixture of water and organic solvents like methanol or acetonitrile. When a sample is injected, the compounds interact with the stationary phase based on their hydrophobicity. Compounds with higher hydrophobicity will elute slower, while more polar compounds will elute faster.
