The catalytic mechanism of LDH involves several key steps. The enzyme binds to both pyruvate and NADH, positioning them in a way that facilitates the transfer of electrons from NADH to pyruvate. This process involves the formation of a ternary complex, followed by a hydride transfer from NADH to pyruvate, resulting in the formation of lactate and NAD+. The enzyme's active site contains key residues that stabilize the transition state and lower the activation energy of the reaction.
