Crosslinking: Cells are treated with formaldehyde to crosslink proteins to DNA, preserving the protein-DNA interactions. Shearing: The crosslinked chromatin is then sheared into smaller fragments, typically using sonication or enzymatic digestion. Immunoprecipitation: An antibody specific to the protein of interest is used to precipitate the protein-DNA complexes. Purification: The crosslinks are reversed, and the DNA is purified from the protein-DNA complexes. Analysis: The purified DNA is then analyzed to determine the sequences bound by the protein, often using techniques like PCR, qPCR, or sequencing.
