How can RNase A be modified to enhance its catalytic properties?
Various strategies can be employed to enhance the catalytic properties of RNase A. Site-directed mutagenesis can be used to introduce specific amino acid substitutions that improve substrate binding or catalytic efficiency. Chemical modifications, such as PEGylation, can enhance enzyme stability and reduce susceptibility to inhibitors. Additionally, fusion proteins combining RNase A with other functional domains can be engineered to create multifunctional catalysts with improved performance.
