Proton transfer can be studied using a variety of experimental and computational techniques. Spectroscopic methods like NMR, IR, and UV-Vis can provide insights into the intermediates and transition states involved in proton transfer. Kinetic studies can help determine the rate-limiting steps and the influence of proton transfer on the overall reaction rate. Computational methods, including quantum chemical calculations and molecular dynamics simulations, can provide detailed mechanistic insights and help visualize proton transfer pathways.
