Several strategies can be employed to minimize column bleeding:
Temperature Control: Operating the column at lower temperatures can reduce the rate of stationary phase degradation. Stationary Phase Selection: Using thermally stable stationary phases can help minimize bleeding. Column Conditioning: Proper conditioning of the column before use can stabilize the stationary phase and reduce bleeding. Carrier Gas Purity: Using high-purity carrier gases can prevent the introduction of contaminants that might exacerbate column bleeding.
