Studying ribozymes involves a combination of experimental and computational techniques:
- X-ray Crystallography and NMR: These techniques are used to determine the three-dimensional structures of ribozymes, providing insights into their catalytic mechanisms. - Mutagenesis Studies: By creating specific mutations in ribozymes, researchers can identify critical regions and nucleotides necessary for catalytic activity. - In Vitro Selection and Evolution: These methods allow for the identification and optimization of ribozymes with desired catalytic properties from large libraries of RNA sequences.
