Kinases are studied using various biochemical and biophysical techniques to understand their catalytic mechanisms. Techniques include:
- X-ray Crystallography: Provides detailed structural information on kinase-substrate complexes. - NMR Spectroscopy: Offers insights into the conformational dynamics of kinases. - Kinetic Assays: Measure the rate of phosphorylation to determine the catalytic efficiency and substrate specificity of kinases. - Mutagenesis Studies: Involve altering specific amino acids in the kinase to study their role in catalysis and substrate binding.
