The development of chaperone therapies involves several key steps: 1. Target Identification: Identifying the protein or enzyme that is misfolded or unstable. 2. High-Throughput Screening: Screening large libraries of small molecules to find candidates that can bind and stabilize the target protein. 3. Optimization: Refining the identified molecules to improve their binding affinity and specificity. 4. Preclinical and Clinical Testing: Evaluating the efficacy and safety of the optimized chaperones in preclinical models and clinical trials.
